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KMID : 0603820080140020083
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Journal of Experimental & Biomedical Science
2008 Volume.14 No. 2 p.83 ~ p.89
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Enhanced Susceptibility of Human Squamous Cell Carcinoma to Photodynamic Therapy Combined with Administration of Cisplatin
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Ahn Jin-Chul
Chung Phil-Sang
Park Byung-Keon
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Abstract
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We have compared the antitumor effect of photodynamic therapy (PDT), using 5-aminolevulinic acid (ALA) as the photosensitizer, combined with cisplatin (CDDP) on AMC-HN3 human squamous cell carcinoma. AMC-HN3 cells were cultured and then incubated with various concentrations of CDDP and ALA. 632 nm diode laser was given at followed by incubation for 24 hours. The evaluation of cell viability was done by MTT assay. In vivo CDDP was injected intraperitoneally 24 hours prior to PDT. The anti-tumor effects of each treatment were measured by tumor volume change. Cell viability were 44.29% for the cisplatin-mediated chemotherapy group , 77.22% for ALA-PDT group, and 15.06% for the Combination therapy group. In vivo, the antitumor effect of photodynamic therapy was enhanced by combination of Cisplatin-mediated chemotherapy. Photodynamic therapy combined with administration of Cisplatin appears to enhance antitumor effect and to be a useful treatment modality.
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KEYWORD
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Photodynamic therapy (PDT), 5-aminolevulinic acid (ALA), Cisplatin (CDDP), AMC-HN3 cell, Carcinoma
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